YM-47522, a Novel Antifungal Antibiotic Produced by Bacillus sp-I. Taxonomy, Fermentation, Isolation and Biological Properties

YM-47522, a novel antibiotic, was isolated from the culture broth of Bacillus sp. YL-03709B. The antibiotic was purified by centrifugal partition chromatography and ODS column chromatography. It exhibited potent in vitro antifungal activity especially against Rhodotorula acuta and Pichia angusta (MIC : 0.05 and 0.75 μg/ml, respectively). It also showed moderate or weak antifungal activity against Candida albicans and Cryptococcus neoformans (MIC : 25 and 6.25 μg/ml, respectively), whereas it was inactive against filamentous fungi and bacteria.