Photo-enhanced antibacterial activity of polydopamine-curcumin nanocomposites with excellent photodynamic and photothermal abilities.

Abstract Background and objective Photodynamic therapy (PDT) and photothermal therapy (PTT) have gradually become options for select anti-tumor and antibacterial treatment . The combination of PDT and PTT show great research value, which may greatly improve the curative effect. The aim of the present study was to prepare a compound system of polydopamine and curcumin (PDA-Cur nanocomposites) with excellent antibacterial effect towards Gram-positive and Gram-negative bacteria. Methods Dopamine hydrochloride was oxidized and self polymerized in alkaline condition to form PDA-Cur nanocomposites. The structure and morphology of PDA-Cur were characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), laser scattering microscopy (LSM), ultraviolet spectrophotometer (UV-vis), infrared spectroscopy (IR) and fluorescence emission spectrometer. Using 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), 1,3-diphenylbenzofuran (DPBF) and 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) were used to detect the production of reactive oxygen species (ROS). The thermal stability of PDA-Cur nanocomposites was investigated by temperature rising test. The antibacterial effect of PDA-Cur was determined by plate counting technique using Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) as models. In addition, the stability and antibacterial mechanism of PDA-Cur were investigated. Finally, the biocompatibility was evaluated by cytotoxicity and hemolysis tests. Results The compound system of polydopamine and curcumin was successfully prepared, which showed improved stability compared with Cur. The consumption of DPBF by the singlet oxygen produced by PDA-Cur was as high as 80%. In the heating test, the highest temperature increased to 59 °C, which contributed to the photodynamic and photothermal inactivation of bacteria. PDA-Cur nanocomposites showed good antibacterial activity against S. aureus and E. coli. Under 405 nm light, the bactericidal rate of PDA-Cur against S. aureus can reach 100% at a low concentration of 10−4 nM, and that against E. coli was 100% at 1 nM. Under 405 + 808 nm light, the bactericidal rate of PDA-Cur against E. coli enhanced to 100% at 0.1 nM. In addition, PDA-Cur had low cytotoxicity and negligible hemolytic activity, showing good biocompatibility. Conclusion PDA-Cur nanocomposites had good photodynamic effect, photo thermal conversion ability and biocompatibility. Compared with free Cur, the antibacterial activity of PDA-Cur was significantly improved, and the antibacterial effect with combined light was stronger than that of free Cur. Therefore, the construction of PDA-Cur nanocomposites have confirmed that the combination of PDT and PTT can greatly improve the antibacterial effect and reach bactericidal effect at low concentration, which provides a strategy for the design of next generation antimicrobial agents.