[Effects of ezrin silencing on pancreatic cancer cell line Panc-1].
2012
Objective To explore the effects of ezrin silencing on pancreatic cancer cell line Panc-1.Methods Pancreatic cancer cell line Panc-1 was transfected with ezrin silencing plasmid.The proliferation and the cell cycle status were determined by CCK-8 assay and flow cytometry analysis,respectively.Cellular membrane protrusions/microvilli formation were visualized by scanning election microscopy.Colony formation assay was used to determine the cell anchor-independent growth ability in vitro.Trans-filter migration and invasion assays were performed with 8 μm pore inserts in a 24-well BioCoat chamber with/without Matrigel.Results Ezrin silencing decreased cellular protrusions/microvilli formation,anchorage-independent growth,cell migration and invasion,but had no effects on cell proliferation in vitro and cell cycle,in pancreatic cancer cell line Panc-1.Conclusion Ezrin expression affects the cellular protrusions/microvilli formation,anchorage-independent growth,cell migration and invasion in pancreatic cancer cell line Panc-1.
Key words:
Pancreatic neoplasms ; Cytoskeleton ; Tumor cell,cultured
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