Development of an amplified luminescent proximity homogeneous immunoassay kit for detecting human hepatitis B virus e antibody
2011
Objective To develop an amplified luminescent proximity homogeneous immunoassay(AlphaLISA) kit for the detection of human hepatitis B virus e antibody(HBeAb) .Methods The neutralizing and competitive inhibition method was used to develop the AlphaLISA kit for detection of serum HBeAb.Results The working range of the kit was 0.003-16 NCU/ml with a sensitivity up to 0.003 NCU/ml.The intra-and inter-assay coefficient of variation was 5.3% and 6.8%,respectively.The kit showed no cross-reaction with HBcAb,and comparison of the detection results with those of a commercially available Elecsys HBeAb kit(Roche) for 136 samples showed a correlation coefficient of 0.961.Conclusion The AlphaLISA kit for HBeAb detection meets the clinical?requirements for detection HBeAb in human serum.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
0
Citations
NaN
KQI