A study on the use of phenylacetyl disulfide in the solid‐phase synthesis of oligodeoxynucleoside phosphorothioates

Sulfurization of protected [i.e., 2-cyanoethyl, methyl and 2-(4-nitrophenyl)ethyl] internucleosidic phosphite triesters can be conveniently executed with the easily accessible reagent, phenylacetyl disulfide (1). High-quality oligodeoxynucleotides containing predetermined combinations of natural and phosphorothioate linkages can be obtained via a solid-phase approach by using reagent 1 and 2-(4-nitrophenyl)ethyl-protected phosphoramidites.