DETECTION OF BAT CORONAVIRUS AND SPECIFIC ANTIBODIES IN CHESTNUT BAT (SCOTOPHILUS KUHLII) POPULATION IN CENTRAL TAIWAN

Bats can serve as natural reservoirs for severe acute respiratory syndrome (SARS) and Middle East respiratory syndrome coronavirus (MERS-CoV). Investigating the prevalence of bat CoV is critical for assessing the risks of the outbreaks of emerging CoV. Chestnut bats (Scotophilus kuhlii) were captured in this study for detecting the partial RNA-dependent RNA polymerase (RdRp) gene in their feces through reverse transcription polymerase chain reaction (RT-PCR) and antibodies to the nucleocapsid (N) protein of bat CoV through western blotting (WB) analysis. Three recombinant N protein fragments (N1, N2, N3) of the isolated Scotophilus bat CoV/CYCU-S1/TW/2013 were expressed by Escherichia coli. WB analyses were performed with bat serum samples and the sera of a patient who recovered from a SARS-CoV infection. Fragment N2 contained a highly conserved motif among CoVs whereas N1 and N3 protein fragments were specific to the S. kuhlii bat CoV. A total of 32 fecal and 19 serum samples were collected in Changhua C...