TPX2 gene-silencing inhibits the proliferation and invasion of human colon cancer SW480 cells

Objective: To investigate the effects of targeting protein for Xenopus kinesin-like protein 2 (TPX2) gene-silencing on the human colon cancer SW480 cells, and to explore its possible mechanism.Methods: After transfection with the recombination vector-targeting TPX2 gene-silencing pMagic4.1-shRNA-TPX2, the abilities of proliferation and invasion of SW480 cells were detected by MTT method and Transwell chamber assay, respectively. The expression levels of TPX2, vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) mRNA and protein were measured by RT-PCR and Western blotting, respectively.Results: The abilities of proliferation and invasion of SW480 cells after transfection with the recombination vector-targeting TPX2 gene-silencing pMagic4.1-shRNA-TPX2 were lower than those of negative control (SW480 cells transfected with the pMagic4.1-shRNA-NC) and blank control cells (SW480 cells without any transfection) (all P < 0.01). The expression levels of TPX2, VEGF and MMP-9 mRNA and protein of SW480 cells after transfection with pMagic4.1-shRNA-TPX2 were lower than those in the negative control and the blank control cells (all P < 0.01).Conclusion: TPX2 gene-silencing can obviously inhibit the proliferation and invasion of SW480 cells. This effect maybe related to down-regulation of expressions of VEGF and MMP-9 gene. DOI:10.3781/j.issn.1000-7431.2016.11.051