Two methods for isolating wild boar (Sus scrofa ferus) DNA.

DNA isolation, one of the first steps in many molecular genetics studies, has to be efficient both in terms of yield and quality, but also in terms of costs. The present paper compares the DNA yield and purity of two inexpensive laboratory alkaline lysis protocols for wild boar DNA isolation, providing a comparison between two different starting materials: whole blood and hair bulbs. Both protocols yielded DNA quantities considered adequate for PCR-based analyses. Whole blood, as a starting material, was statistically superior to hair bulbs both in terms of yield and purity.