Characterization of a powdery mildew resistance gene in wheat breeding line 10V-2 and its application in marker-assisted selection

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a serious disease of wheat (Triticum aestivum L.) throughout the world. Host resistance is the most effective and preferred means for managing this disease. 10V-2, a wheat breeding line with superior agronomic performance, shows broad-spectrum seedling resistance to powdery mildew. Genetic analysis demonstrated that its resistance was controlled by a single dominant gene, tentatively designated Pm10V-2. This gene was localized at near the documented Pm2 locus on chromosome 5DS using the simple sequence repeat (SSR) marker Cfd81. To saturate the the marker map of Pm10V-2, more markers were developed, and used for bulked segregant RNA-Seq (BSR-Seq) and marker tagging. Two single nucleotide polymorphism (SNP) markers, Swgi047 and Swgi064, three expressed sequence tag (EST) markers, Swgi007, Swgi029 and Swgi038 and one SSR marker, Swgi066, were polymorphic between the resistant and susceptible bulks and showed tightly linked to the Pm10V-2 g...