The significance of high-risk human papillomavirus DNA testing and liquid-based cytology in the diagnosis of high-grade cervical lesions

Objective To evaluate the significance of high-risk human papillomavirus(HR-HPV) DNA testing and liquid-based cytology in screening high-grade cervical lesions.Methods A total of 800 cases had liquid-based cytology test and HR-HPV DNA testing by hybrid capture Ⅱ in which 280 cases received cervical biopsy because of abnormal cytology.Results ①The overall prevalence of HPV infection was 36.8%.Two hundred and ninety-four patients were HPV positive with the mean age of(39±9) years,while 506 patients were HPV negative and their mean age was(38±8) years;the former was significantly younger than the later(P0.05).②In the 280 cases,the rate of high-grade cervical lesions were 45.0%(128/280)and 52.2%(109/209)in people with abnormal cervical cytology above ASC-US and LISL,respectively.The rate was increased with aggravation of cervical cytology(P0.05).③In 127 high-grade cervical lesions the rate of HPV infection was 92.9%.The rare was 89.2% in CINⅡand 93.6% in CINⅢ respectively.④Among 71 patients with cervical cytology of ASC-US in the 280 cases,the rate of high-grade cervical lesions was 23.9% and was higher in HPV positive group(P0.05).Conclusion HR-HPV DNA testing binding with liquid-based cytology test can help improve the screening of high-grade cervical lesions and it has great value.It is the best choice for the screening of cervical cancer.