CLONING AND EXPRESSION OF A GENE ASSOCIATED WITH HL60 CELL APOPTOSIS INDUCED BY INHIBITION OF POLYAMINE BIOSYNTHESIS

Objective: To clone the gene associated with apoptosis induced by an inhibitor of polyamine biosynthesis, α-difluoromethylornithine (DFMO). Methods: The differential subtraction screening was used for gene cloning from cDNA library of HL60 cells treated by DFMO. Northern blot, morphological observation, FCM assays and ladder map of DNA electrophoresis were performed. Results: The transfecting gene expression and activity of inducing apoptosis in the cells transfected from recombinant plasmid containing the cloned fragment df4 was proved. Conclusion: It is suggest that df4 gene cloned in the study could be a gene regulating apoptosis of HL60 cells.