Analysis of β-cryptoxanthin from yellow pigmented marine bacterium Erythrobacter sp. kj5

The objectives of this study were to isolate and analyze β-cryptoxanthin from yellow pigmented marine bacterium Erythrobacter sp. KJ5. The pigments from Erythrobacter sp. KJ5 were extracted from cells with methanol:acetone (7:3, v/v). β-cryptoxanthin standard was isolated from citrus fruit peel using 100% acetone and purified by high-performance liquid chromatography (HPLC) using a C30 column. The existence of β-cryptoxanthin in Erythrobacter sp. KJ5 was determined based on spectral properties and co-chromatography analyses after adding the standard β-cryptoxanthin. The co-chromatography results showed that peak 5 at retention time 33.32 min sharply spiked without changing maximum wavelengths (λmax)at 453 and 480 nm after adding the standard β-cryptoxanthin. These results likely conclude that peak 5 was β-cryptoxanthin.