Chicken poly(ADP-ribose) polymerase. Complete protein sequence deduced from cDNA,comparison with mammalian enzyme sequences

In the past few years human poly(ADP-ribose)polymerase (pADPRP) cDNA (Uchida et al., 1987, Kurosaki et al., 1987) as well as the bovine (Saito et al., 1990), mouse (Huppi et al., 1989) and part of the rat (Thibodeau et al., 1989) cDNAs have been cloned revealing an extensive aa sequence conservation in mammals. To further study the interspecies conservation, we isolated a 1.8 kb human pADPRP cDNA clone from an oligo (dT) primed lgt10 cDNA library (Walter et al., 1985). This clone encodes the entire automodification domain of the enzyme and a great part of the NAD binding domain. Cross hybridization of chicken genomic DNA and mRNA with this human probe has been obtained in low-stringency conditions (Ittel et al., 1989). Using this 3’ probe and another one covering the 5’ region of the pADPRP human cDNA from nucleotide 1 to 697 (kindly provided by Dr G. Gradwohl), and called 5’ probe, we cloned and sequenced the chicken pADPRP cDNA. Its deduced aa sequence has been compared with the known mammalian as sequences.