Neuron primary culture purification method

2015 
The invention belongs to the technical field of animal cell culture, and specifically relates to a neuron primary culture purification method which comprise the following steps: providing nerve tissues, performing enzymatic hydrolysis and digesting tissues, separating nerve cells with a density gradient centrifugation method, inoculating nerve cells, separating nerve cells by using the differential velocity adhesion characteristic, purifying nerve cell with cytosine arabinoside (Ara-C) to obtain neurons. The neuron primary culture purification method largely shortens primary culture purifying period of DRG neurons, largely promotes the purification of the neurons, guarantees cell activities, facilitates cell culture and growth, and lays a good foundation on the further experiments.
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