An integrated bioprocess for xylanase production from agriculture waste under open non-sterilized conditions: Biofabrication as fermentation tool

Abstract Indiscriminate disposal of the solid waste generated from various agricultural practices cause detrimental effects on the environment. Utilization of the waste biomass for production of value-added products through biotechnological intervention not only helps to combat environmental pollution but also contributes significantly to the economy. In this study, a biofabrication software (fermentor tool) was applied to analyze statistics of xylanase production under solid non-sterile fermentation conditions. In solid substrate fermentation wheat bran, banana peel, millet waste, and sugarcane bagasse were evaluated. Maximal xylanase titer of 10545 U/g dry matter was obtained when wheat bran was used as a substrate (4 days incubation at 50 °C and pH value of 8.0). Partially purified xylanase was applied in saccharification of lignocellulosic materials to assess its potential application in a lignocellulosic biorefinery. In this context, the precision of predicted mathematical model was evaluated and fermentation provided a good prediction of the concentration of cell, substrate and product. Second, open non-sterilized fermentation method was established to save sterilization cost that seems to be a very economical and practically applicable to the enzyme industry.