Preliminary investigation of quantified detection of airborne T-2 and HT-2 toxins in one henhouse

A total of 40 air samples were collected by AGI-30, 20 in November in 2009 and 20 in April in 2010 from one henhouse in Dalian, which is a seashore city belonging to marine climate in northeast of China. The henhouse feed more than five thousand layer chickens, and its floor area is 500 m2. Since a long exposure to the henhouse air, twelve drinking water samples of these 2 months were also collected to detect the level of the two toxins. High-performance liquid chromatography with fluorescence detection and 1-antroylnitrile (1-AN) as labeling reagent after immunoaffinity cleanup was used for the detection of T-2 and HT-2 toxins. The results of this work showed that the concentration of airborne T-2 and HT-2 toxins was higher in the sites 500 m away from the doorway than that 250 m away from the doorway, and no toxins were detected at gate. Toxins collected in April were much higher than that in November. The highest concentration of T-2 and HT-2 in henhouse was 9.39 and 27.83 ng/m3, respectively.