A Simple and Efficient Culture of Leaflets for Plant Regeneration in Guineagrass (Panicum maximum)

A simple and efficient culture for plant regeneration was established from the leaflet tissue of Panicum maximum. The vegetative leaflets were excised from terminal tillers arising at the end of stolons and cultured for callus formation on Murashige and Skoog (MS) medium supplemented with 2, 4-dichlorophenoxyacetic acid. After 30 days of culture, compact callus formed. And after 60 days, white embryogenic calli were induced with wave-like-shapes. When the embryogenic calli were transferred onto MS medium supplemented with kinetin and giberellic acid for regeneration, they developed and formed multiple shoots. The rooted plants were obtained within 4 months. All of the regenerants were successfully grown to maturity.