Improved performance of PACE 2 with modified collection system in combination with probe competition assay for detection of Chlamydia trachomatis in urethral specimens from males
1994
textabstractThe Gen-Probe PACE 2 assay (GP) in combination with a modified collection
system was compared with cell culture (CC) for the detection of Chlamydia
trachomatis in urethral specimens from males. Analysis of discordant
results was performed by PCR. The modifications, i.e., application of a
more rigid swab type and a 50% reduction in the amount of transport
medium, were made to improve the sensitivity of the assay. By using the
modified GP on 302 urethral specimens from males, a sensitivity of 89.5%
and a specificity of 100% were determined. In addition, performance of a
probe competition assay on all GP samples with a result > 0.6 and < 1.0
times the cutoff factor (gray zone) detected three more true-positive
samples. The sensitivity of GP in combination with the probe competition
assay increased to 94.9%, with a specificity of 100%. This was identical
to the performance of CC. The modified GP offers a very sensitive and
specific alternative to CC.
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