Studies on extraction of bifidobacterium plasmid by mutanolysin from Streptomyces globisporus fermentation fluid

Objective To find an economic method to extract the plasmid from Bifidobacterium.Method Bifidobacterium plasmid was extracted by mutanolysin from Streptomyces globisporus which could lyse the wall of bifidobacterium.The result was compared with that by lysozyme.Result Both mutanolysin and lysozyme could successfully lyse the wall of bifidobacterium and extract the plasmid from Bifidobacterium.Conclusion Mutanolysin from Streptomyces globisporus is an economic strategy to the plasmid extraction for Bifidobacterium.