Supporting Information for Proteomics DOI 10.1002/pmic.200800271

/ 1.0% Triton X-100/ 0.75% SDS/ 10 mM Tris, pH8.0). Unbroken cells and debris were removed by centrifugation (4,500 x g for 15 min). The cleared supernatant was incubated with 1 ml of Ni-NTA agarose (Qiagen) overnight at room temperature. The resin was packed into a column and washed with 10 ml of wash buffer A, 10 ml of wash buffer B (8 M urea/ 500 mM NaCl/ 0.1 mM NaH