Interspecies Differences on Ovarian Parameters between Black Bengal Goat and Indigenous Bengal Sheep in View of In vitro Maturation

Black Bengal goat and indigenous Bengal sheep are the most important small ruminants in Bangladesh. To characterize the ovarian parameters and to study interspecies variation this research was conducted. Ovaries were collected from local slaughterhouse and categorized as left/right and CL-present/absent. After evaluation in terms of weight, length, width, height, number of atretic and non-atretic follicle in; Cumulus Oocyte Complexes were collected from 90% of the ovaries and remaining 10% were subjected to histological study. After tissue preparation and staining with Haematoxylene and Eosin stain, the diameter of primordial, primary and secondary follicles; thickness of follicular layers of graffian follicles were measured. It was observed that left and right ovaries were equally active to normal physiological and/or ovarian activity, as the parameters of both ovaries in both species were similar (P>0.05). The weight, length, width and height of CL present ovaries were significantly lower (P<0.05) but the number of non-atretic follicles, number of normal and total COCs were significantly higher (P<0.05) than CL present type in both species. The results of species comparison showed that all the parameters in sheep were significantly higher (P<0.05) than those of goat. In histological study, it was observed that the diameter of primary and secondary follicle, thickness of theca interna and theca externa in graffian follicle in goat were significantly higher than those of sheep.