Method for building high-yield 5-aminolevulinic acid escherichia coli engineering strains

The invention discloses a method for building high-yield 5-aminolevulinic acid Escherichia coli engineering strains, and belongs to the field of metabolic engineering and micro-biological fermentation. On the basis that key enzyme glutamy tRNA reduction enzyme and glutamyl aminotransferase which are 5-aminolevulinic acid C5 synthesis paths are overexpressed through carriers pACYCDuet-1, uroporphyrinogen III synthase (UROS) hemD codes and coproporphyrinogen III oxidase (CPO) hemF codes from an escherichia coli heme biosynthetic pathway are independently expressed with pCDFDuet-1 or are expressed together, and recombination strains are constructed. By means of fermentation verification, hemD or hemF is expressed separated, hemD and hemF are expressed together, and the ALA yield is obviously improved.