Biotransformation of (−)-Verbenone by Human Liver Microsomes

The biotransformation of (−)-verbenone was investigated with human liver microsomes by using GC-MS. Regioselective biotransformation was observed when (−)-verbenone was incubated with the liver microsomes. (−)-10-Hydroxyverbenone was formed from (−)-verbenone of kinetic analysis showed that the K m and V max values for the hydroxylation of (−)-verbenone by liver microsomes from three human samples, HG-70, HG-56 and HG-23, were 1.1 mM and 4.8 nmol/min/nmol P450, 0.6 mM and 2.1 nmol/min/nmol P450, and 2.8 mM and 4.6 nmol/min/nmol P450, respectively.