Inhibitory role of smart nano-trifattyglyceride of Moringa oleifera-root in ovarian cancer by attenuating FSHR - c-Myc axis

Abstract: Background and Aim Epithelial ovarian cancer has the deadliest prognosis amongstgynaecological cancers, warranting an unmet need for newer drug targets. Based onits anticancer as well as abortifacient potential, Moringa oleifera Lam. root washypothesized to have some implications in follicle stimulating hormone receptor(FSHR) dependent cancers like epithelial ovarian cancer. Experimental procedure Effect of Moringa oleifera Lam. root extract (MRE) was studied in epithelial ovariancancer cell line through in vitro studies viz. MTT assay, clonogenic assay, cell cycleanalysis, flow cytometry, western blot analysis, immunocytochemical analysis of FSHRand c-Myc expression and in vivo studies viz. effect of MRE in mice model of ovariancarcinoma. The structure of the active compound of MRE was elucidated followingsolvent extraction, purification through column chromatography, preparative TLC andbioactivity guided structural identification through 1H-NMR, 13C-NMR, DEPT-135, ESIMS,FT-IR spectrophotometry, UV-vis-NIR spectrophotometry and DFT study. Results and Conclusion Crude MRE displayed cytotoxic activity, induced apoptosis, andattenuated expression of FSHR and c-Myc in ovarian cancer cell line OAW42. MREalso attenuated expression of CD31, FSHR, and c-Myc in tumour xenograft mousemodel. Finally, the active compound purified from ethyl acetate-n-hexane subfraction ofMRE, that attenuated viability of ovarian carcinoma cell lines and reduced FSHR and c-Myc expression, was identified as a naturally hydrated-trifattyglyceride, showing aDFT-optimized folded amphipathic structure for easy transportation through hydrophilicand hydrophobic regions in a biological system, indicating its immense therapeuticrelevance in epithelial ovarian carcinoma.