Comparison of HRP and Lucifer Yellow(LY) as Indicators in Permeability Evaluation of in vitro Blood Brain Barrier Model

Objective To compare the using effect of HRP and Lucifer Yellow(LY) as the indicators in permeability evaluation of in vitro blood brain barrier model(BBBM).Methods Brain capillary endothelial cells(BCECs) and astrocytes(As) collected from one-day old Wistar rats were co-cultured to build up non-contact BBBM.The permeability coefficients(Pe) of HRP and LY to BBBM were measured by permeability test.Results Non-contact BBBM matching 12 and 24-well plate separately were built up.Permeability test showed that the Pe values of HRP both in 12 and 24-well models were lower than those of LY.The Pe values of HRP group were(0.64±0.05)×10-3 cm/min and(1.67±0.07)×10-3 cm/min,and Pe values of LY group were(1.08±0.16)×10-3 cm/min and(3.03±0.13)×10-3 cm/min in 12 and 24-well model respectively.Conclusion Both HRP and LY are the effective indicators for quantitative permeability evaluation of in vitro BBBM.In view of its safety and simplicity in use,LY should be the first choice for BBBM permeability test.