The concept of the portable spectrometer for fast assessment of skin engraftment via exogenous and endogenous fluorophores

Today the most important problem of a transplantation is a rejection of healing skin tissue. The reason of a skin rejection is a high level of inflammation reaction and a slow rate of neoangeoginesis. A lot of methods exist for imaging of tissue healing extent, unfortunately, all of them have some drawbacks. Laser induced fluorescence is a non-invasive method which provides ambulatory and fast diagnosis. The concept was created and optimal parameters of spectral device were selected based on the experiment results. The non-invasive spectral device will allow determining a state of a healing skin and rate of skin tissue engraftment or rejection by its spectroscopic properties analysis using aluminum phthalocyanine nanoparticles (nAlPc). These nanoparticles are spectroscopically sensitive to inflammation reactions and begin to fluoresce while interacting with immune cells in inflamed tissue. The operation principle of developed device based on analysis of diffuse reflected light from a skin area. The device consists of the six red laser diodes. The red range laser irradiation allows dedicating autofluorescence of biological tissue components such as lipopigments, porphyrins. The fluorescence intensity of exogenous fluorophores helps identifying the degree of transplant engraftment because it is correlate with the inflammatory reactions intensity in a skin. End users will be burn centers, medicine facilities for monitoring of a postoperative sutures engraftment. It can also be used at home to assess the healing of small wounds.