Deep-brain 2-photon fluorescence microscopy in vivo excited at the 1700-nm window
2019
Here we demonstrate deep-brain 2-photon fluorescence microscopy in
mouse in vivo, excited at the 1700 nm window. Through signal versus
power measurement, we show that indocyanine green (ICG) is a promising
2-photon fluorescent dye excitable at the 1700 nm window. In order
to excite ICG circulating in the vasculature in the deep brain, we employ
a circular-polarization soliton self-frequency shift technique to generate
energetic femtosecond pulses at 1617 nm. Combining the labeling and
laser technologies, we achieve a record 2-photon fluorescence brain
vasculature imaging depth of 2000 μm in vivo. Both the
effective attenuation length measurement and signal-to-background ratio
measurement indicate that we have reached the theoretical depth limit in
2-photon fluorescence microscopy.
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