Thermosensitive bFGF-Modified Hydrogel with DentalPulp Stem Cells on Neuroinflammation of Spinal Cord Injury
2020
Acute
spinal cord injury (SCI) induces severe neuroinflammation,
which increases intermediary filaments and neurodegeneration. Previous
studies have shown that a basic fibroblast growth factor (bFGF) and
dental pulp stem cells (DPSCs) contribute to a protective effect on
injured neuronal cells, but the mechanism of SCI repair is still unclear.
In this study, in situ heparin (HeP) hydrogel injection containing
bFGF and DPSCs (HeP-bFGF-DPSCs), as well as in vitro studies of bFGF
and DPSCs, proved an effective control over inflammation. The in vivo
application of HeP-bFGF-DPSCs regulated inflammatory reactions and
accelerated the nerve regeneration through microtubule stabilization
and tissue vasculature. Our mechanistic investigation also showed
that bFGF-DPSCs treatment inhibited microglia/macrophage proliferation
and activation. Furthermore, HeP-bFGF-DPSCs prevented microglia/macrophage
activation and reduced proinflammatory cytokine release. In this paper,
we discovered that bFGF and DPSCs worked together to attenuate tissue
inflammation of the injured spinal cord, resulting in a superior nerve
repair. Our results indicated that a thermosensitive hydrogel delivering
bFGF and DPSCs could serve as a promising treatment option for spinal
cord injuries.
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