The transition dipole moment representation and spectral phasors

Spectral phasors are currently based on fluorescence spectra in the wavelength domain. However, this is a problem when using spectral phasors to quantify emitters since wavelength represented spectra are not proportional to emitter population. Here we show the effect of unmixing using spectra in their transition dipole moment representation for a theoretical two component system and on a real seven component system.