Pathway of dephosphorylation of myo-inositol hexakisphosphate by a novel phytase from Candida krusei WZ-001

Abstract myo -Inositol hexakisphosphate (InsP 6 ,) was hydrolyzed by a novel phytase purified from Candida krusei . The chemical structures of the product (InsP 1–5 ) were identified by an NMR technique. The data indicate that the products from the enzyme catalytic reaction in sequence are d / l -Ins(1,2,4,5,6)P 5 , d / l -Ins(1,2,3,5,6)P 5 , d / l -Ins(1,2,5,6)P 4 , d / l -Ins(1,2,5)P 3 , d / l -Ins(1,2)P 2 and d / l -Ins(2)P 1 .