Analytical Method Development for Bisphenol a in Tuna by Using High Performance Liquid Chromatography-UV

Abstract Bisphenol A (BPA) detection in food has gained research interest in recent years. This attention is based on BPA characteristic, easy to migrate and potentially hazard for human, and their usage in many packaging products polluting food and environment. In this research, a reverse phase high liquid chromatography coupled with UV detector (HPLC-UV) was developed for determination BPA in tuna. The analytical method was validated based on the following criteria: linearity, precision, repeatability, limit detection, and recovery. Excellent linear correlation with R 2 = 0.9992 was observed over the range of 2 to 250 mg/kg. The detection limit was calculated to be 0.8 mg/kg, while the limit of quantification (LOQ) value of the validated method was measured to be 1.5 mg/kg. Intra-day and intermediate precision (RSD, %) were 3.42 and 3.50, respectively. Good recoveries of 89.84% with RSD% = 5.41 were obtained at level concentration 32 mg/kg. The proposed method was found to be suitable and precised for the determination of BPA in tuna by using HPLC-UV.