Systematic Evaluation of O-glycosylation Mediated Enhancement and Modulation of the Cel7A Carbohydrate Binding Module Function

3 Introduction 4 i. Crystalline Cellulose Affinity Enhancement by O-­‐glycosylation of Cel7A CBM 6 ii. O-­‐glycan Modulation of Cel7A CBM Cellulose Specificity 9 iii. Lignin Non-­‐productive Adsorption by Cel7A CBM and Modulation by O-­‐glycosylation.12 Materials and Methods 14 Results and Discussion 20 i. Synthesis and Characterization of CBM Glycoforms 20 ii. Cel7A CBM O-­‐Mannosylation Confers Adsorption Enhancement Towards Crystalline Cellulose 20 iii. O-­‐Mannose Modulation of Cel7A CBM Specificity for Model Cellulose Systems........26 iv. CBM Glycoform Non-­‐productive Adsorption on Kraft Lignin 30 Concluding Remarks and Future Directions 33 Acknowledgements 35 References 36 Appendix I 48 Appendix II 49