Protocols for RLGS Gel Production

RLGS can be performed using various enzyme combinations (restriction enzymes EL-EB-EC). The labeling method must be chosen according to the shape of the recognition site of enzyme EL. In addition, the reaction buffers used in each process should be changed, accompanied by variation of the enzyme. Here, protocols for two good representative enzyme combinations, combination 1 (NotI-PvuII-PstI) and combination 2 (PacI-EcoRV-MboI), are shown.