Reinforcing properties of pups versus cocaine for fathers and associated central expression of Fos and tyrosine hydroxylase in mandarin voles (Microtus mandarinus).

Abstract The reciprocal interaction of pups and cocaine on reward effects in rodent mothers is known. However, it remains unclear whether such effects are apparent in father-offspring bonding. The mandarin vole ( Microtus mandarinus ) is a monogamous rodent with a high level of paternal care. We investigated the reinforcing properties of pups on vole fathers using a conditioned place preference paradigm across the postpartum period and looked for interactions and differences between the reinforcing effects of pups and cocaine. We also measured neuronal Fos and tyrosine hydroxylase (TH) expression underlying the preferences of fathers for pups or cocaine. Our data showed that fathers developed strong preferences for pups at various times (postnatal day 5–9, 13–17 and 19–23) without cocaine conditioning. Fathers showed a reduced preference for pups following simultaneous conditioning with cocaine. Although they preferred cocaine over postnatal day (PND) 5–9 pups, this preference was not detected for PND 13–17 pups. Fathers preferring cocaine exhibited an increase in Fos-immunoreactive neurons in the accumbens, medial nucleus of the amygdala, cingulate cortex, medial preoptic area and ventral tegmental area and had more TH-IR neurons in the ventral tegmental area compared to fathers preferring PND 5–9 pups. These results showed that similar to cocaine, mandarin vole pups elicit significant reward value to their fathers, but that paternal motivation is impaired by cocaine. A preference for cocaine over pups arose from the release of more dopamine and activation of a greater number of neurons within specific reward-associated neuronal subsets.