Chemical composition and antimicrobial activity of methanolic extract of Trichoderma sp. growing wild in Tunisia.

Abstract The chemical composition of methanol extract of Trichoderma sp. Mycelium was analysed by GC-FID and GC-MS. 16 compounds were identified representing 93.6 % of its total composition. Methyl 9,12,15-octadecatrienoate (51.8 %) and phytol (10.5 %) were found to be the main compounds. The antimicrobial activity of the extract was determined against five Gram-positive and Gram-negative bacteria and five phytopathogenic fungi. The tested extract exhibited an interesting antibacterial activity against all bacteria tested except the Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, but no antifungal activity was observed.