The Determination of Creatinine in Human Urine by Capillary Zone Electrophoresis with Photodiode Array Detection

The application of capillary zone electrophoresis (CZE) to the separation and determination of creatinine in human urine with photodiode array detection was described. Optimum conditions for the separation were established. The effect of pH of the background buffer on the existence state of creatinine was investigated. Photodiode array detection permitted the rapid identification of creatinine in the samples analysed. By selecting 234 nm as monitoring wavelength, the interference from urea in urine was eliminated. By selecting a suitable pH, the interferences from other substances in urine were also eliminated. The linear range, detection limit, precision and recovery of the method were investigated.