Correlation between Guttata Severity and Thickness of Descemet’s Membrane and the Central Cornea

ABSTRACTPurpose: To characterize and correlate guttata severity, Descemet’s membrane thickness (DMT), central cornea thickness (CCT) in corneas with guttae using specular microscopy and spectral-domain optical coherence tomography (SD-OCT) and test the Doheny Image Reading Center (DIRC) specular microscopy-based corneal guttata severity scale.Methods: Forty-nine eyes of 49 patients with guttata and 36 eyes of age-matched of 36 normal controls were enrolled in the study. Three images of the central cornea and four of the peripheral cornea (inferior, superior, nasal and temporal) of each eye were taken using the Konan NSP-9900 specular microscope. A volume scan of the central cornea cross-section was collected on each eye using the Heidelberg Spectralis SD-OCT. The density of endothelial guttata based on specular images was graded on a 0–4 scale, and the Descemet’s membrane thickness (DMT) and central corneal thickness (CCT) were manually measured by two trained graders.Results: The DIRC corneal guttata sev...