Measuring Both Glucose Uptake and Myosin Heavy Chain Isoform Expression in Single Rat Skeletal Muscle Fibers

: Glucose uptake by skeletal muscle is important for metabolic health. Because skeletal muscle is composed of multiple fiber types that have differing metabolic and contractile properties, studying glucose uptake in whole muscle tissue does not elucidate differences at the cellular level. Here, we describe a procedure that enables the measurement of both glucose uptake and fiber type (by myosin heavy chain isoform expression) in individual rat epitrochlearis muscle fibers.