Fe(III) reduction due to low pe+pH contributes to reducing Cd transfer within a soil-rice system

Abstract Effect of Fe redox state caused by low soil pe+pH levels on Cd uptake by rice is unclear. Rice grown in pots of Cd-contaminated paddy soil were subjected to different irrigation regimes: flooding, intermittent flooding (Int-FL), and sustained soil moisture at 70% water holding capacity (WHC). Results showed low pe+pH (5.52 and 7.09) in flooding treatment significantly increased relative abundances of Fe-reducing bacteria (FeRB) (6.29% and 4.51%), especially members within the Clostridium, Geobacter and Desulfuromonadia genera. Stimulation of FeRB activity induced Fe(III) reduction and increased Fe2+ content in flooded soils, which promoted Cd sequestration in low-crystalline fraction of IP (IP-Feh-Cd) and Cd bonded to amorphous Fe-oxides (amFeox-Cd). The 24.9%-62.4% higher amFeox-Cd content was the important factor for 20.4%–44.2% lower CaCl2-extractable Cd content in flooding treatment than those in other treatments. Soil submergence reduced Cd uptake by rice at tillering and booting stages, the critical periods of Cd transport in the soil-rice system, which was attributed to the increases in dissolved Fe2+ and IP-Feh-Cd contents and decrease in CaCl2-Cd content. Therefore, maintaining flooding during the tillering and booting stages may be an effective strategy to reduce Cd uptake by rice cultivated in Cd-contaminated soil.