[Isolation and preliminary identification of stem-like cells in pituitary adenoma].

Objecitve To isolate and identify the pituitary adenoma stem-like cells from pituitary adenoma tissue.Methods Total RNA was prepared with 42 cases of pituitary adenoma tissue samples frozen in liquid nitrogen,the expression of Nestin was detected by RT-PCR.Tumor spheres were cultured in serum-free conditions and the immunefluorescence were used to detect the expression of stem cell markers such as Nestin,Sox2.Results The positive rate of Nestin mRNA expression was 88.1%(37/42).The cultured tumor spheres were found positive for Nestin,Sox2 by immunefluorescence detection.Conclusion Stem cell markers are expressed in the pituitary adenoma tissue and the pituitary adenoma stem-like cells can be cultured in serum-free condition.