Fluorescence Lifetime Imaging Microscopy Using a Tunable Continuum Source and a Nipkow Disk Confocal Microscope

David M. Grant,Egidijus Auksorius,Damian Schimpf,Daniel S. Elson,Christopher Dunsby,Jose Requejo-Isidro,Ian Munro,Mark A. A. Neil,Paul M. W. French,Patrick Courtney

Fluorescence Lifetime Imaging Microscopy Using a Tunable Continuum Source and a Nipkow Disk Confocal Microscope

2005

David M. Grant
Egidijus Auksorius
Damian Schimpf
Daniel S. Elson
Christopher Dunsby
Jose Requejo-Isidro
Ian Munro
Mark A. A. Neil
Paul M. W. French
Patrick Courtney

Multi-beam confocal sectioned fluorescence lifetime imaging microscopy is demonstrated using a Yokogawa spinning disk. The single-photon excitation source is a supercontinuum generated from a Ti:sapphire seeded photonic crystalline fibre.

Keywords:

Scanning confocal electron microscopy
Two-photon excitation microscopy
Light sheet fluorescence microscopy
Nipkow disk
Fluorescence-lifetime imaging microscopy
Microscope
Super-resolution microscopy
Microscopy
Optics
Materials science

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