Genetic characterization of G II.12 norovirus in Beijing from 2008 to 2009

Objective To reveal the genetic characteristics of G Ⅱ.12 Norovirus strains isolating from stool samples of adults with diarrhea in Beijing during 2008-2009.Methods RdRp,ORF2,ORF3 and ORF1/ORF2 overlap region were respectively amplified by primers using RT-PCR.The products were purified,cloned,sequenced and then aligned,phylogenetie and recombinant analyzed by softwares of DNAStar,M EGA and SimPlot.Results According to the phylogenetic analysis,11 strains belonged to G Ⅱ.g in the RdRp region,while GⅡ.12 in the ORF2 and ORF3.SimPlot analysis further confirmed the 11 strains were recombinant strains (G Ⅱ.g [RdRp]/G Ⅱ.12 [capsid]).Conclusion G Ⅱ.12 Norovirus prevailing in Beijing and other regions of the world belonged to the same strain,and we identified the genetic characteristics of G Ⅱ.12 Norovirus in Beijing. Key words: Norovirus ;  Diarrhea;  Gastroenteritis ;  Recombination, genetic