Cloning, characterization and mapping of human SPIN to human chromosome 9q22.1-22.3

Spindle is a specific substructure in cell during meiosis and gamete maturity division and its structure and function directly influence the development, differentiation, growth and reproduction of individuals. Recent researches also show that mouse Mos/MAPK pathway is tightly related to spindlin phosphorylation. We report that (i) a novel gene encoding a putative human spindlin (SPIN) has been cloned, which is highly homologous to mouse Spin; (ii) tissue expression pattern ofSPIN shows thatSPIN is highly expressed as a 4.8 kb transcript in ovary, testis, heart, brain, kidney, pancreas, placenta and spleen, also with a specific 2.0 kb transcript in testis, but there is no hybridization band in thymus and small intestine, and (iii)SPIN has been mapped to human chromosome 9q22.1-22.3 by Radiation Hybrid Mapping. This novel gene was registered in GenBank asSPIN with accession number AF087864.