Polyurethane sheet impregnated with Arabinogalactan can lead to increase of attachment of promastigotes and Amastigote of Leishmania major (MRHO/IR/75/ER) by GP63 and HSP70 genes

Abstract The aim of this study was to investigate the effect of polyurethane sheet (PUS) and polyurethane sheet impregnated with Arabinogalactan (PUSIAG) on the cell attachment and viability of Promastigotes and Amastigotes of Leishmania major (MRHO/IR/75/ER), and mouse macrophages, and its whole skin cells (WSCs). In a sterile condition, 10 mL of Arabinogalactan 5% w / v was poured into a falcon. Then, a piece of PUS was placed inside it, and incubated at 37 °C for 24 h. Next, it was washed, and cut. Then, one piece of PUS and PUSIAG was separately added to 1 mL of cell suspension (Promastigotes, Amastigote, and WSCs), and then incubated for 1, 2, 3, and 4 days at 37  ° C. After incubation times, the quantity of adhered cells was counted, and cell viability was measured by MTT assay. Also, for WSCs and macrophages, the expression of integrin, fibronectin and GAPDH was investigated, and for Promastigotes and Amastigotes, the expression of GP63 , Cpb , and 18s rRNA was measured. This study showed that with increase of exposure time, the percentage of attached cells was increased. There was a significant difference between attached cells to PUSIAG and PUS in case of Promastigotes and Amastigotes. It seems that Promastigotes and Amastigotes have higher interest to PUSIAG than WSCs and Macrophages. Also, this study showed with increase of exposure time, the percentage of viable cells was decreased. There were significant differences between cell viability of Promastigotes and Amastigotes when exposed to PUSIAG and PUS, especially in long time incubation. Also, when incubation time was increased the relative expression of integrin and fibronectin in WSCs and macrophages, and GP63 and HSP70 in Promastigotes and Amastigotes were increased.