THE DEVELOPMENT OF A POINT OF CARE CREATININE MEASUREMENT USING DISPOSABLE READY TO USE MICROCHIP CAPILLARY ELECTROPHORESIS

We report on the determination of creatinine in human serum using a point-of-care device suitable for the use by untrained operators, the Medimate Multireader®. This device is based on electrophoretic separation and conductivity detection and its prefilled microfluidic chip has a single opening for sample introduction. The device was originally developed for the determination of lithium in blood [1]. Creatinine determination at a level of quantification of 300 μM was realized by optimizing the background electrolyte (BGE) pH and concentration, and the injection and separation potentials and time. This limit of quantification is in the relevant range for the detection of renal failure [2].