II. Methodologies and Medicinal Chemistry Strategies to Discover and Develop New TreatmentsChapter 4:Drug Assay Methodology in Leishmaniasis: From the Microplate to Image Analysis

Drug assays are critical to the anti-leishmanial discovery process. In this chapter, we look at the ways that different methodologies of drug assays contribute to the acquisition of essential data on drug efficacy and safety against live Leishmania infections. We outline essential considerations for designing robust drug screening assays, covering both old and new models of Leishmania infection and technologies for the detection of drug activity of new compounds.