Embryo rescue and hybrid identification of sweet orange(Bingtang) and citron

In order to obtain zygotic seedlings from hybrid of sweet orange(Bingtang) and citron, a experiment was conducted by taken Bingtang as female parent and citron with resistance to canker as male parent. Embryo rescue technology was firstly used to obtain filial generation, and then the genomic DNA of parents and their hybrids were amplified by PCR using screened simple sequence repeats(SSR) primers, and zygotic seedlings of next generation were further identified. The results showed that twelve zygotic seedlings were identified from the 359 obtained seedlings by SSR markers.