The research of separation and purification technology for effective parts from Zhizichi decoction by macroporous absorption resina

Objective To optimize separation and purification technology of Zhizichi decoction by macroporous resina. Methods The content of total iridoid glycosides and total isoflavones were determined by ultraviolet spectrophotometry. Taking the content of total iridoid glycosides and total isoflavones as indexes, the effect of the concentration of sample solution, medicinal herbs on the amount of sample, concentration and volume of eluent was investigated by single factor test. Results The optimum separation and purification technology was as following: the concentration of sample solution was 0.1 g/ml, the volume ratio of resin to material drug was 2:1, the adsorption time was 2 h, and the sample was firstly eluted with 1 BV water, then 6 BV 20% ethanol and 60% ethanol, and the eluent was collected. Conclusion This optimized separation and purification technology was reasonable and stable, and it could be extended to large-scale production applications.