De novo biosynthesis of alpha-zingiberene from glucose in Escherichia coli

2021 
Abstract Alpha-zingiberene, a functionally important active component of ginger, is used extensively in various applications in the fields of medicine, food, and agriculture. In this study, alpha-zingiberene was de novo biosynthesized from glucose using metabolically engineered Escherichia coli as hosts. First, the alpha-zingiberene synthase, VvPNaZin, was heterologously expressed in an E. coli strain. The resultant recombinant strain, LZ020, produced 0.012 ± 0.002 mg/L of alpha-zingiberene. Next, ERG20 was overexpressed in LZ021 to increase the farnesyl pyrophosphate (FPP) content, which enhanced the yield of alpha-zingiberene to 0.041 ± 0.017 mg/L. Finally, the heterologous mevalonate (MVA) pathway was introduced to increase the supply of dimethylallyl diphosphate (DMAPP) and isopentenyl diphosphate (IPP) precursors, which induced LZ022 to produce 0.764 ± 0.048 mg/L of alpha-zingiberene, representing a 63.7-fold increase compared to that produced by the LZ020 strain. Thus, the current study established a technique that facilitates microbial synthesis of alpha-zingiberene directly from glucose, thereby providing methodology which enables sustainable production of alpha-zingiberene.
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