Efficiency of ISSR marker in assessing the genetic diversity of wild and cultivated Hedysarum polybotrys Hand. Mazz

AbstractGenetic variation of cultivated and wild populations plays an important role in maintaining the population gene polymorphism and developing future management strategies. Hedysarum polybotrys is an important economic plant in Gansu Province, China. This paper was the first to investigate and analyse the genetic diversity between wild and cultivated H. polybotrys. The results of the ISSR-PCR revealed that 479 (98.97%) of the 484 ISSR loci tested by 17 primers were polymorphic. The change of PPL was 92.98–93.80%. The species level of the alleles, the effective number of alleles, the Nei’s gene diversity and the Shannon’s information index were 1.9897, 1.6006, 0.3502 and 0.5228, respectively. The high–low sequence of the genetic diversity of the wild population was greater than that of the cultivated population. The genetic distance and genetic identity between the cultivated and wild populations were 0.0488 and 0.9524. AMOVA analysis indicated that genetic variation mainly occurred among populations ...